Fmoc-Ala-Wang Resin (100–200 Mesh): Preloaded Resin for C-Terminal Alanine Peptides
Fmoc-Ala-Wang Resin is a preloaded polystyrene resin used in Fmoc-based solid-phase peptide synthesis (SPPS). It is functionalized with Fmoc-protected L-alanine attached to the resin via a Wang linker, which is acid-labile and ideal for producing peptides with a C-terminal free carboxylic acid (-COOH). The resin simplifies synthesis by eliminating the need for manual first amino acid coupling and ensures uniformity across peptide batches.
This resin is ideal for synthesizing peptides with L-alanine at the C-terminus, which is a common amino acid in bioactive peptides and serves as a neutral, small, nonpolar residue.
Specifications
Matrix: Polystyrene cross-linked with ~1% DVB
Linker: Wang linker (acid-labile phenyl ester)
Preloaded Amino Acid: Fmoc-L-Alanine
Particle Size: 100–200 mesh (75–150 µm)
Loading: Typically 0.4–0.8 mmol/g
Cleavage: TFA (standard acidolysis)
Compatible Chemistry: Fmoc/tBu peptide synthesis
Final Peptide Terminus: C-terminal carboxylic acid
Key Features
Preloaded with Fmoc-Ala: Eliminates the need for first amino acid coupling
Fmoc-Compatible: Integrates seamlessly into automated or manual SPPS workflows
Acid-Labile Wang Linker: Clean release of peptide upon TFA cleavage
High Resin Stability: Resistant to degradation under synthesis conditions
Reproducible Peptide Yields: Preloading ensures consistency
Applications
Synthesis of peptides with C-terminal alanine
Development of alanine-scanned analogs
Structure-activity relationship (SAR) studies
Small-scale research and large-scale automated synthesis
Protease substrate studies where alanine is the leaving group
Cleavage Conditions
Reagent: TFA (95%) with scavengers (e.g., water, TIS, EDT)
Time: 1–2 hours at room temperature
Result: Peptide with a free C-terminal –COOH
Handling & Storage
Store in a dry, sealed container, protected from moisture and light
Swell prior to use in DCM or DMF
Compatible with standard SPPS instrumentation and protocols
Related Products
| Product | C-Terminus | Use Case |
|---|---|---|
| Fmoc-Ala-Wang Resin | –COOH | Peptides with C-terminal Ala |
| Fmoc-Gly-Wang Resin | –COOH | Peptides with C-terminal Gly |
| Wang Resin (unloaded) | –COOH | Custom loading of first amino acid |
| Rink Amide Resin | –CONH₂ | Peptides requiring C-terminal amide |
Conclusion
Fmoc-Ala-Wang Resin (100–200 mesh) is a high-quality, preloaded resin designed for the efficient synthesis of peptides terminating in L-alanine with a free carboxylic acid. Its Fmoc compatibility, ease of use, and consistent performance make it a standard choice for peptide chemists working on alanine-rich sequences or SAR libraries.
Phone : +49 1512 4714765 ( Germany )
Phone : +1 (702) 381-3042 ( USA )
Email : [email protected]
Address : Cunostraße 56, 14193 Berlin. Germany
Address : 7373 Rowlett Park Dr, Tampa, FL 33610, United States.