H-Gly-2-ClTrt Resin: Preloaded Chlorotrityl Resin for Peptides with C-Terminal Glycine Acids
H-Gly-2-ClTrt Resin is a preloaded, acid-labile resin based on 2-chlorotrityl chloride (2-ClTrt) polystyrene, functionalized with N-unprotected glycine (H-Gly). It is designed for Fmoc solid-phase peptide synthesis (SPPS) to provide peptides with a C-terminal glycine and free carboxylic acid (-COOH). The 2-ClTrt linker allows for extremely mild acid cleavage, making it especially valuable for synthesizing acid-sensitive peptides, protected intermediates, or fragments for ligation.
This resin eliminates the need for initial amino acid loading, ensuring high reproducibility and convenience in workflows focused on glycine-terminated sequences.
Specifications
Matrix: Polystyrene cross-linked with ~1% DVB
Linker: 2-Chlorotrityl chloride (2-ClTrt)
Preloaded Amino Acid: Glycine (H-Gly, free amine)
Loading: Typically 0.3–0.8 mmol/g
Particle Size: 100–200 mesh (approx. 75–150 µm)
Compatible Chemistry: Fmoc/tBu peptide synthesis
Cleavage Conditions: Very mild acidic (e.g., 1–5% TFA/DCM)
Final Peptide Terminus: C-terminal glycine with –COOH
Key Features
Preloaded with H-Gly: Saves time, ensures reproducibility
Mild Cleavage: Ideal for acid-sensitive sequences or side-chain-protected fragments
Fmoc-Compatible: Fully suited for standard SPPS workflows
Clean Release: Minimal side-products or tarring during cleavage
Ideal for Fragment Coupling: Useful for native chemical ligation or segment condensation
Applications
Peptide synthesis with C-terminal glycine residues
Protected peptide fragments for convergent synthesis
Acid-sensitive or labile peptide sequences
Native chemical ligation (NCL) precursors
Peptide synthesis with on-resin cyclization or modifications
Cleavage Protocol Options
| Cleavage Reagent | Conditions | Result |
|---|---|---|
| 1–5% TFA in DCM | 5–30 min at RT | Protected peptide–COOH (partial) |
| 95% TFA (standard) | 1–2 hrs at RT | Fully deprotected peptide–COOH |
Mild conditions preserve side-chain protections, allowing for advanced synthesis strategies.
Handling & Storage
Store dry, under inert gas if long-term storage is needed
Swell in DCM or DMF prior to synthesis
Moisture-sensitive: protect from air and humidity
Compatible with manual or automated SPPS systems
Comparison with Related Resins
| Resin | C-Terminus | Cleavage Strength | Use Case |
|---|---|---|---|
| H-Gly-2-ClTrt Resin | –Gly–COOH | Very mild (1–5% TFA) | For protected fragments, glycine-terminated peptides |
| Wang Resin | –COOH | Strong (95% TFA) | General-purpose C-terminal acid peptides |
| Rink Amide Resin | –CONH₂ | Strong (95% TFA) | Peptides with amide termini |
| 2-ClTrt Resin (unloaded) | –COOH | Very mild (1–5% TFA) | Custom loading with sensitive linkers |
Conclusion
H-Gly-2-ClTrt Resin is a top-tier choice for peptide chemists seeking high-purity glycine-terminated peptides, especially when working with acid-sensitive sequences or protected fragments. Its mild cleavage profile, preloaded convenience, and broad SPPS compatibility make it an essential tool in both research and production-scale synthesis.
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